Research Article

INFLUENCE OF AUXINS ON ROOTING STEM CUTTINGS OF MORINGA OLEIFERA LAM IN DRY AND WET SEASONS

1 Department of Biology Education, Federal College of Education (Technical), Potiskum, Yobe State
2 Department of Plant Science and Biotechnology, University of Nigeria Nsukka, Enugu State
3 Department of Biological Sciences, Federal University Wukari, Taraba State
* Corresponding author: maibukars@gjids.org.ng
Published: Jul, 2015
Pages: 89-97

Abstract

The study was designed to evaluate the influence of three types of auxins: Indole Butyric Acid (IBA), Naphthalene Acetic Acid (NAA) and, Indole Acetic Acid (IAA) at four concentrations (0mg/l, 100mg/l, 300mg/l and 500mg/l) and two seasons (dry and wet) on rooting mature stem cuttings of Moringa oleifera. The experiment was 3×4×2 factorial laid in a completely randomized design with three replicates. The findings of this study show that, M oleifera can be propagated vegetatively by rooting the stem cuttings with auxins. The results of the developmental phases of the stem cuttings prior to rooting significantly (P>0.05) showed variations due to auxin concentrations and seasons. Stem cuttings treated with the higher concentration of the three auxins (500ppm of IBA, NAA and IAA) showed that wet season stem cuttings sprouted earlier (18-27 days after planting, DAP) than the dry season stem cuttings (24-30 DAP). The results also showed that auxin application and seasons influenced higher bud development into leaves in the wet than in the dry season (2.0% - 83.4% wet season and, 10% - 56.08% dry season). It was also observed that, untreated (control) stem cuttings and those treated with 100mg/l and 300mg/l did not root in the dry season trial. While lateral roots were observed on stem cuttings across the treatments in the wet season, only those treated with 500mg/l of the auxins produced tap roots with IAA proving most effective. Therefore, rooting stem cuttings of M. oleifera with 500mg/l of IAA during the wet season is recommended.

References

  1. Anbarassan, P., Sreeja. K. V., Kalaiselvi, S., Parvatham, A. and Vedamuthu, P. G. B. (2001). Our Moringa Experience: An Overview. Current Science 45 (21): 769-779.
  2. Araya, H. T., Soundy, P., du Toit, E. S., Madau. F. N. (2007). Influence of Cutting Position, Medium, Hormone and Season on Rooting of Bush Tea (Athrixia phylicoides) Stem Cuttings. Medicinal and Aromatic Plant Science and Biotechnology 1: 243-252.
  3. Awoleye, F. (1991). Vegetative Propagation of Some Indigenous Fruit Trees in the Southern Guinea Savanna: Preliminary Results. Nigerian Horticultural Science Journal 1: 21-26.
  4. Bassiil, N. V., Proebsting, W. M., Moore, L. W. and Lightfoot, P. (1991). Propagation of Hazel Nut Stem Cuttings Using Agrobacterium rhizogenes. Horticultural Science 26: 15-20.
  5. Erez, A. (1984). Improving the Rooting of Peach Hardwood Cuttings Under Field Condition. Horticultural Science 19(2): 245-247.
  6. Fahey, J. W. (2005). Moringa oleifera: A Review of the Medical Evidence for its Nutritional, Therapeutic and Prophylactic Properties. Part 1. Trees for Life Journal 1: 5.
  7. Foldi, N., Makkar, H. P. S. and Becker, K. (2001). The Potential of Moringa oleifera for Agricultural and Industrial Uses. In: “The Miracle Tree: The Multiple Attributes of Moringa” (Ed. Lowell J Fuglie). CTA, USA.
  8. Gomez, K. A. and Gomez, A. A (1984). Statistical Procedures for Agricultural Research. 2nd Edition. John Wiley & Sons. 680pp.
  9. Griffith, B. G. (1940). Effect of Indolebutyric Acid, Indole Acetic Acid, and Alpha Naphthalene-Acetic Acid on Rooting of Cuttings of Douglas-Fir and Sitka Spruce. Journal of Forestry 38: 496-501.
  10. Hartmann, H. T. and Kerster, D. E. (1983). Plant Propagation, Principles and Practice. 4th Ed. Prentice Hall, Englewood Califf. N.J.
  11. Klein, J. D., Cohen, S. Hebbe, Y. (2000). Seasonal Variation in Rooting Ability of Myrtle (Myrtus communis L. ) Cuttings. Scientia Horticulturae 83: 71-76
  12. Nzekwe, U. (2002). Studies on Some Aspects of the Biology and Ecology of Irvingia wombolu syn. I. gabonensis var excelsa. Ph.D Thesis, Department of Botany, University of Nigeria. 155 p.
  13. Nzekwe, U., Onyekwelu, S. S. C. and Unach, V. C (2002). Improving the Germination of Irvingia gabonensis. Nigerian Journal of Horticultural Science 7(2): 48-52.
  14. Nzekwe, U. (2006). The Role of Plant Bioresearches in the Development of Biotechnology in Nigeria. Invited Paper, 19th Annual Conference, Biotechnology Society of Nigeria. 12-18 Nov., 2006. University of Jos.
  15. Ofori, D. A., Newton, A., Leakey, R. B. and Grace, J. (1996). Vegetative Propagation of Milicia excelsa by Leafy Stem Cuttings: Effect of Auxin Concentration, Leaf Area and Rooting Medium. Forest Ecology and Management 84(1-3): 39-48.
  16. Price, M. (2007). The Moringa Tree. Echo Technical Note. 19 p.
  17. Proebsting, W. M. (1984). Rooting Douglas-Fir Stem Cuttings: Relative Activity of IBA and NAA. Horticultural Science 19(6): 854-856.
  18. Puffy, S., Kwena, W. M., Elsa, S. D., Phatutsani, N. M. and Hintsa, T. A. (2008). Influence of Cutting Position Medium, Hormone and Season on Rooting of Fever Tea (Lippia javanica L.) Medicinal and Aromatic Plant Science and Biotechnology 2(2): 114-118.
  19. Puri, S. (1990). Rooting of Stem Cuttings of Casuarina equisetifolia and their Nodulation. The International Tree Crop Journal 6: 51-55.
  20. Rajangam, J., Azahakia-Manavalan, R. S., Thangarj, T., Vijayakumar, A. and Muthukrishan, N. (2001). Status of Production and Utilization of Moringa in Southern India. In: The Miracle Tree The multiple Attributes of Moringa (Ed. Lowell J. Fuglie). CTA. USA.
  21. Singh, B. K. (2004). Biodiversity Conservation and Management. Mangal Deep Publishers. Juniper. 586 p.
How to Cite

S, M. B., U, N., & E, O. (2015). INFLUENCE OF AUXINS ON ROOTING STEM CUTTINGS OF MORINGA OLEIFERA LAM IN DRY AND WET SEASONS. Gashua Journal of Irrigation and Desertification Studies, 1(1), 89-97. https://doi.org/10.67390/gjids.2015.nanj8ush

M. B. S, N. U, and O. E, "INFLUENCE OF AUXINS ON ROOTING STEM CUTTINGS OF MORINGA OLEIFERA LAM IN DRY AND WET SEASONS," Gashua Journal of Irrigation and Desertification Studies, vol. 1, no. 1, pp. 89-97, July 2015. doi: 10.67390/gjids.2015.nanj8ush

Share this article:
Facebook X / Twitter LinkedIn