Research Article

Hydrolysate Yield, Surface Hydrophobicity and Amino acid Properties of Desert Date (B.aegyptiaca Del) Protein Hydrolysates

Ogori, A. F*, Jackson, V., Girgih, T. A.
Published: Nov, 2025
DOI: 10.67390/gjids.2025.trbia8v0
Pages: 110-122

Abstract

Desert date, called B.aegyptiaca Del commonly wild in desert regions of the world. The seed was processed into Aduwa Protein Concentrate (APC) and afterward hydrolyzed using two combined endogenous Pepsin-Pancreatin (PP) proteases and two different exogenous proteases (Alcalase and flavourzyme) to respective hydrolysates and analyzed using standard methods. The result of the analysis revealed that Alcalase hydrolysate had a higher protein yield (99%) compared to APC (62.22%), PP (83.23%) and flavourzymes (62.90%). The protein content and surface hydrophobicity of Alcalase hydrolysate were above the reference control and the other hydrolysates. The excellent surface-related properties exhibited by the Alcalase hydrolysate were below the APC reference control. The PP hydrolysate had higher content of hydrophobic amino acid and sulphur-containing amino acid (40.19%, 7.04%) compared to Alcalase (38.92%, 6.69%), Flavourzyme (37.43%,6.35%), and APC (39.97%, 6.95%) samples. The hydrolysates of PP and Alcalase have better amino acid residues and surface-related properties and could be employed as ingredients in the nutraceutical and functional food industries. Desert date called B.aegyptiaca Del commonly wild in desert regions of the world. The seed was processed into Aduwa Protein Concentrate (APC) and afterword hydrolyzed using two combined endogenous Pepsin-Pancreatin (PP) proteases and two different exogeneous proteases (Alcalase and flavourzyme) to respective hydrolysates and analyzed using standard methods. Result of analysis revealed that Alcalase hydrolysate had higher protein yield (99%) compared to APC (62.22%), PP (83.23%) and flavourzymes (62.90%). The protein content and surface hydrophobicity of Alcalase hydrolysate were above the reference control and the other hydrolysates. The excellent surface related properties exhibited by the Alcalase hydrolysate was below the APC reference control. The PP hydrolysate had higher content of hydrophobic amino acid and sulphur containing amino acid (40.19%, 7.04%) compared to Alcalase (38.92%, 6.69%), Flavourzyme (37.43% ,6.35%), and APC (39.97%, 6.95%) samples. The hydrolysates of PP and Alcalase has better amino acid residues and surface related properties and could be employed as ingredients in the nutraceutical and functional food industries.

Keywords

Desert date concentrate yield enzymes Hydrolysate.

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How to Cite

F, O. A., V., J., & A., G. T. (2025). Hydrolysate Yield, Surface Hydrophobicity and Amino acid Properties of Desert Date (B.aegyptiaca Del) Protein Hydrolysates. Gashua Journal of Irrigation and Desertification Studies, 3(1), 110-122. https://doi.org/10.67390/gjids.2025.trbia8v0

O. A. F, J. V., and G. T. A., "Hydrolysate Yield, Surface Hydrophobicity and Amino acid Properties of Desert Date (B.aegyptiaca Del) Protein Hydrolysates," Gashua Journal of Irrigation and Desertification Studies, vol. 3, no. 1, pp. 110-122, November 2025. doi: 10.67390/gjids.2025.trbia8v0

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